Journal: PLOS One

Article Title: Targeted transferosomal delivery of cetirizine: A new approach to alopecia management

doi: 10.1371/journal.pone.0347648

Figure Lengend Snippet: Insulin-like growth factor-1 (IGF-1) and vascular endothelial growth factor (VEGF) mRNA and protein expression levels in different groups.

Article Snippet: IGF-1 and VEGF protein levels in hair bulbs were measured using a Mouse & Rat IGF-I/IGF-1 ELISA Kit (Quantikine; R&D systems, Minneapolis, MN, USA) and a Mouse VEGF ELISA Kit (Quantikine; R&D systems), respectively, according to the manufacturer’s instructions [ ].

Techniques: Expressing

Journal: Journal of Functional Biomaterials

Article Title: A Borophosphate Glass Doped with Cobalt Oxide Improves Skeletal Muscle Structure and Function in Myopathic Mice

doi: 10.3390/jfb17030155

Figure Lengend Snippet: TRIM enhances vascularity. ( A , B ) Representative images of TA cross-sections in WT and D2. mdx mice at 14 dpt. Laminin + immunostaining (white) identified basal laminae surrounding myofibers; CD31 + immunostaining (green) identifies endothelial cells as a measure of vascularity. Laminin intensity in the merged images was reduced to enhance visualization of CD31 + staining. Yellow arrows identify CD31 + staining in merged image. ( C ) Summary values ( n = 4–5/group) for area occupied by ECs were calculated as: total EC area/number (#) of fibers. ( D ) Summary values ( n = 4–5/group) for EC-to-myofiber ratio were calculated as: # of EC puncta/# of myofibers. Summary values presented for 14 dpt. Angiogenic factors are increased with TRIM. ( E ) VEGF concentration in whole-muscle TA homogenates ( n = 5/group) probed for by ELISA. Summary values are means ± SEM; comparisons made vs. WT and vehicle controls by 2-Way ANOVA, p < 0.05 = significant. Scale bars = 100 μm.

Article Snippet: Samples of muscle supernatant (50 μL; referred to above) were analyzed to determine VEGFA protein concentration using a Quantikine ELISA (Cat.# MMV00, R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s instructions.

Techniques: Immunostaining, Staining, Concentration Assay, Enzyme-linked Immunosorbent Assay

Journal: Journal of Functional Biomaterials

Article Title: A Borophosphate Glass Doped with Cobalt Oxide Improves Skeletal Muscle Structure and Function in Myopathic Mice

doi: 10.3390/jfb17030155

Figure Lengend Snippet: TRIM enhances vascularity. Representative images of TA cross-sections in D2. mdx Saline (Top) and D2. mdx TRIM (Bottom) mice. ( A ) A 70 dpt and ( E ) 140 dpt. Laminin+ immunostaining (white) identified basal laminae surrounding myofibers; CD31 + immunostaining (green) identifies endothelial cells as a measure of vascularity. Laminin intensity in the merged images was reduced to enhance visualization of CD31 + staining. Yellow arrows identify CD31 + staining in merged image. Summary values ( n = 7–8/group) for area occupied by ECs were calculated as: total EC area/number (#) of myofibers. Summary values presented for ( B ) 70 dpt and ( F ) 140 dpt. Summary values ( n = 7–8/group) for EC-to-myofiber ratio were calculated as: # of EC puncta/# of myofibers, presented for ( C ) 70 dpt and ( G ) 140 dpt. Angiogenic factors are increased with TRIM. VEGF concentration in whole muscle TA homogenates probed for by ELISA at ( D ) 70 dpt and ( H ) 140 dpt. ( n = 7–8/group); summary values are means ± SEM. Comparisons made vs. vehicle controls by two-tailed Student’s t -test; p < 0.05 = significant. Scale bars = 100 μm.

Article Snippet: Samples of muscle supernatant (50 μL; referred to above) were analyzed to determine VEGFA protein concentration using a Quantikine ELISA (Cat.# MMV00, R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s instructions.

Techniques: Saline, Immunostaining, Staining, Concentration Assay, Enzyme-linked Immunosorbent Assay, Two Tailed Test